Isotype-specific changes in the amount of beta-tubulin RNA in synchronized tobacco BY2 cells. |
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| Authors: | S Okamura K Okahara T Iida M Ozaki S Asano M Morita T Imanaka |
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| Institution: | Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan. okamura@ms.toyama-mpu.ac.jp |
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| Abstract: | The 3'-ends of the beta-tubulin cDNA were amplified from tobacco BY2 polyA+ RNA. According to the differences in the predicted amino acid sequence at the extreme C-terminal, they were grouped into three different isotypes, NTB1 in which "EEGDYYEEDEEDLNEA", NTB2 in which "EEEYYEDEEEA QED" and NTB3 in which "DECEYEEEEEYDHEGN" follows the conservative "YQQYQDATAD" sequence. Using unique 3'-untranslated regions as probes, changes in the RNA levels of each beta-tubulin isotype were determined by dot-blot hybridization. The levels exhibited characteristic rhythms in the cell cycle. NTB1 RNA was highest in S phase in comparison to NTB2 RNA level which was highest in late G2. On the other hand, NTB3 RNA level was highest in early G2. |
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