Variations in the morphology of rice plants regenerated from protoplasts using different culture procedures |
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Authors: | Lee Sung-Ho Shon Young Goel Kim Cha Young Chun Hyun Jin Cheong Yong Hwa Kim Zhoo Hyeon Choe Zhin Ryong Choi Young Ju Cho Moo Je |
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Affiliation: | (1) Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Chinju, 660-701, Korea;(2) Department of Horticulture, Gyeongsang National University, Chinju, 660-701, Korea;(3) Department of Agronomy, Gyeongsang National University, Chinju, 660-701, Korea;(4) Department of Food and Nutrition, Silla University, Pusan, 617-736, Korea |
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Abstract: | Rice protoplasts were cultured using 4 different culture procedures such as agarose embedding (AE) without feeder cells and the use of filter membranes (MEM), one layer of nylon mesh (MS1), or a double layer of nylon mesh (MS2) with the inclusion of Lolium multiflorum as feeder cells. The protoplast plating efficiency was highest on the MEM, followed by MS2, MS1 and AE. However, plant regeneration frequencies were highest for MS1, followed by MS2, MEM and AE. The protoclonal plants differed in the morphology of leaves, flowers, spikelets, and panicles in comparison to seed-derived plants. They varied in almost every phenotypic characters evaluated. In many cases, the variation was significantly different in characteristics such as plant height, flag leaf length and width and ratio, and in panicle characteristics such as panicle length, number of primary branches, and number of spikelets per panicle. The number of seeds per panicle was greatly reduced in protoclonal plants when compared with seed-derived control plants. The seeds showed also significant differences in grain length and width in comparison to the control plants. Among the 4 groups of protoclonal plants derived from the 4 different culturing procedures themselves, there were also variations in almost all the phenotypic characteristics assessed. This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | morphology Oryza sativa cv. Taipei 309 plant regeneration rice protoplast culture somatic variation |
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