从云南省蚊体和无名热病人发现另一种新环状病毒

ANOTHER TYPE OF NEW ORBIVIRUSES RECOVERED FROM MOSQUITOES AND PATIENTS WITH FEVER OF UNKNOWN ORIGIN IN YUNNAN PROVINCE

In the summer of 1985, 72 virus isolates were recovered from 10 species of mosquitoes, A. annularis, A. vagus, C. tritaeniorhynchus, C. fus cocephalus,M. uniformis, Ae. albopictuv ,Ae. annandalei, Ae. desmotes, Ae, gardnerii and H. reidi, collected from cowshed and bamboo thicket in Mengding (MD ) of Yunnan. Two other isolates, MD452 & MD459, were also isolated from serum samples of patients with fever of unknown origin( FUO ) collected simultaneously from two hospitals near Mengding. By the methods of enzyme immuno-assay ( EIA ) and neutralization test ( NT ) , MD orbivirus showed no cross-reaction with BANNA orbivirus isolated from patients with FUO, and two other orbivirus isolates, YN187 and Ml49 isolated from mosquitoes. Electron microscopic examination revealed the virions to be morphologically similar to BANNA orbivirus, spherical,without envelope, 66.03?. G8nm in diameter, with double-layered capsids. Although the genomes of MD orbivirus were also composed of 12 double-stranded RNA segments, the profiles of the genomic dsRNA, shared by all the isolates from human and mosquitoes, were remarkably different from those of BANNA orbivirus and other published orbiviru ses. It was suggested that MD orbivirus should be classified as a new member of another serogroup or subgroup of the genus Orbivirus within the family Reoviridae.