Monitoring protein stability <Emphasis Type="Italic">in vivo</Emphasis> |
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Authors: | Email author" target="_blank">Zoya?IgnatovaEmail author |
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Affiliation: | 1.Biotechnologie II,Technische Universit?t Hamburg-Harburg,Hamburg,Germany |
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Abstract: | Reduced protein stability in vivo is a prerequisite to aggregation. While this is merely a nuisance factor in recombinant protein production, it holds a serious
impact for man. This review focuses on specific approaches to selectively determine the solubility and/or stability of a target
protein within the complex cellular environment using different detection techniques. Noninvasive techniques mapping folding/misfolding
events on a fast time scale can be used to unravel the complexity and dynamics of the protein aggregation process and factors
altering protein solubility in vivo. The development of approaches to screen for folding and solubility in vivo should facilitate the identification of potential components that improve protein solubility and/or modulate misfolding and
aggregation and may provide a therapeutic benefit. |
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