Genetic monitoring of populations of <Emphasis Type="Italic">Matthiola Fragrans</Emphasis> (Bunge) using RAPD and AFLP analysis |
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Authors: | N V Khadeeva S V Goryunova A A Kochumova Ye Yu Yakovleva N V Mel’nikova O O Zholobova O I Korotkov A M Kudryavtsev |
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Institution: | 1.Vavilov Institute of General Genetics,Russian Academy of Sciences,Moscow,Russia;2.Volgograd Regional Botanical Gardens,Volgograd,Russia |
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Abstract: | The possibility of using RAPD and AFLP methods for genetic monitoring of populations of Matthiola fragrans (Bunge), a species included in the Red Book of the USSR, was shown for the first time. An analysis of inter- and intrapopulation
and interspecies genome polymorphism was performed. Differences in the genetic structure of Matthiola populations from various geographical collection points were revealed. A simple method of performing RAPD analysis and the
great number of unique markers found in each population compared with the AFLP analysis, as well as the good division of populations
under statistical treatment, allow us to draw the conclusion that using the RAPD method in genetic monitoring of rare and
insufficiently studied species is well founded. |
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