<Emphasis Type="Italic">Cupriavidus</Emphasis> and <Emphasis Type="Italic">Burkholderia</Emphasis> species associated with agricultural plants that grow in alkaline soils |
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Authors: | Paulina Estrada-de los Santos Nora Belinda Vacaseydel-Aceves Lourdes Martínez-Aguilar María Antonia Cruz-Hernández Alberto Mendoza-Herrera Jesús Caballero-Mellado |
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Institution: | 1.Centro de Ciencias Genómicas,Universidad Nacional Autónoma de México,Cuernavaca, Morelos,México;2.Centro de Biotecnología Genómica,Instituto Politécnico Nacional,Reynosa, Tamaulipas,México |
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Abstract: | The presence of Burkholderia, Cupriavidus, and Ralstonia species in northeastern Mexico was investigated. An analysis of the root surrounding soil from different agricultural plants
led to the isolation of Burkholderia and Cupriavidus species but no Ralstonia strains. Most Cupriavidus species were unknown and grouped into two clusters according to ARDRA profiles. The 16S rRNA sequence analysis showed that
the Cupriavidus isolates were highly related among them and with different Cupriavidus species with validated names. However, SDS-PAGE profiles were distinct among the different ARDRA profiles and to other Cupriavidus species examined, suggesting new species in the genus. This shows that Cupriavidus is more widely associated with plants than previously appreciated. The BCC isolate was 99% similar to B. cenocepacia by recA sequence analysis. Additionally, most Cupriavidus strains from the two largest groups grew on media containing up to 0.1 mg/ml of copper, 10.0 mg/ml arsenic and 1.0 mg/ml
zinc. Burkholderia strains grew on media containing up to 10.0 mg/ml zinc, 5.0 mg/ml arsenic and 0.1 mg/ml copper. |
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