Comparison of a randomly amplified polymorphic DNA (RAPD) analysis and ATB ID 32C system for identification of clinical isolates of different Candida species |
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| Authors: | Baires-Varguez Laura Cruz-García Alejandro Villa-Tanaka Lourdes Sánchez-García Sergio Gaitán-Cepeda Luis Alberto Sánchez-Vargas Luis Octavio Quindós Guillermo Hernández-Rodríguez César |
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| Institution: | Facultad de Odontología, Universidad Nacional Autónoma de México (UNAM), 06400 Mexico City, Mexico. baireslaura@yahoo.com.mx |
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| Abstract: | The objective of this work was to compare the usefulness of a randomly amplified polymorphic DNA (RAPD) assay to that of the ATB ID32C kit (bioMérieux, France) for identification of different species of Candida isolated from clinical specimens. The RAPD-PCR patterns obtained with OPE-18 primer for identification of clinical isolates were consistent, and the different independent assays revealed reproduction of the band patterns. RAPD with the OPE-18 primer is a very specific and sensitive method for identification of Candida glabrata, Candida guilliermondii, Candida tropicalis, Candida pelliculosa, Candida albicans, Candida krusei, and Candida lusitaniae. |
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