Selective and quantitative isolation and determination of apomorphine in human plasma |
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Authors: | AWG Essink CPGG Lohuis JT Klein Elhorst WJ Rutten |
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Institution: | Department of Clinical Pharmacy, Medisch Spectrum Twente, Ariënsplein 1, 7511 JX Enschede Netherlands |
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Abstract: | A simple extraction system for the selective and quantitative isolation of apomorphine from human plasma is described. Apomorphine and N-n-propylnorapomorphine were isolated by complex formation between a borate group and the diol group of the apomorphines in an alkaline medium, this in combination with ion-pair formation. The reproducibility and linearity of this extraction method combined with high-performance liquid chromatography with electrochemical detection is excellent. The absolute mean recovery of apomorphine was 100%, the recovery of N-n-propylnorapomorphine was 98%. The detection limit of apomorphine in human plasma in the described system is approximately 0.5 ng/ml. |
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