One of the possible mechanisms for the inhibition effect of Tb(III) on peroxidase activity in horseradish (<Emphasis Type="Italic">Armoracia rusticana</Emphasis>) treated with Tb(III) |
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Authors: | Shaofen Guo Rui Cao Aihua Lu Qing Zhou Tianhong Lu Xiaolan Ding Chaojun Li Xiaohua Huang |
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Institution: | (1) Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Environmental Science, Nanjing Normal University, Nanjing, 210097, People’s Republic of China;(2) The Key Laboratory of Industry Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, People’s Republic of China;(3) Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, People’s Republic of China;(4) State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Science and Biotechnology, Tsinghua University, Beijing, 100084, People’s Republic of China;(5) College of Life Science, Nanjing Normal University, Nanjing, 210097, People’s Republic of China |
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Abstract: | One of the possible mechanisms for the inhibition effect of Tb(III) on peroxidase activity in horseradish (Armoracia rusticana) treated with Tb(III) was investigated using some biophysical and biochemical methods. Firstly, it was found that a large
amount of Tb(III) can be distributed on the cell wall, that some Tb(III) can enter into the horseradish cell, indicating that
peroxidase was mainly distributed on cell wall, and thus that Tb(III) would interact with horseradish peroxidase (HRP) in
the plant. In addition, peroxidase bioactivity was decreased in the presence of Tb(III). Secondly, a new peroxidase-containing
Tb(III) complex (Tb–HRP) was obtained from horseradish after treatment with Tb(III); the molecular mass of Tb–HRP is near
44 kDa and the pI is about 8.80. Thirdly, the electrocatalytic activity of Tb–HRP is much lower than that of HRP obtained
from horseradish without treatment with Tb(III). The decrease in the activity of Tb–HRP is due to the destruction (unfolding)
of the conformation in Tb–HRP. The planarity of the heme active center in the Tb–HRP molecule was increased and the extent
of exposure of Fe(III) in heme was decreased, leading to inhibition of the electron transfer. The microstructure change in
Tb–HRP might be the result of the inhibition effect of Tb(III) on peroxidase activity in horseradish. |
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Keywords: | Tb(III) Peroxidase Microstructure Inhibition mechanism |
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