Complete replication of a eukaryotic virus RNA in vitro by a purified RNA-dependent RNA polymerase |
| |
Authors: | R J Hayes K W Buck |
| |
Affiliation: | Department of Biology, Imperial College of Science, Technology and Medicine, London, England. |
| |
Abstract: | A soluble RNA-dependent RNA polymerase was isolated from Nicotiana tabacum plants infected with cucumber mosaic virus (CMV), which has a genome of three positive-strand RNA components, 1, 2, and 3. The purified polymerase contained two virus-encoded polypeptides and one host polypeptide. Polymerase activity was completely dependent on addition of CMV RNA as template, and the products of reaction were single-stranded (ss) RNA and double-stranded (ds) RNA, corresponding to RNAs 1, 2, and 3, and a subgenomic RNA (RNA 4) derived from RNA 3. The ratio of ssRNA to dsRNA was about 5:1, and the ssRNA was shown to be predominantly the positive strand. This demonstrates the complete replication of a eukaryotic virus RNA in vitro by a template-dependent RNA polymerase. |
| |
Keywords: | |
|
|