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| 硬脂酰-辅酶A脱氢酶基因在食品级乳酸菌中的表达 | |
| 引用本文： | 王腊梅,李世丽,苟克勉,罗玉柱. 硬脂酰-辅酶A脱氢酶基因在食品级乳酸菌中的表达[J]. 生物工程学报, 2012, 28(9): 1106-1117 |
| 作者姓名： | 王腊梅 李世丽 苟克勉 罗玉柱 |
| 作者单位： | 1. 甘肃农业大学动物科学技术学院,甘肃兰州 730070;中国农业大学生物学院农业生物技术国家重点实验室,北京100193 2. 中国农业大学生物学院农业生物技术国家重点实验室,北京,100193 3. 甘肃农业大学动物科学技术学院,甘肃兰州,730070 |
| 基金项目： | 国家转基因生物新品种培育重大专项 (Nos. 2008ZX08008-003, 2009ZX08006-011B), 中央高校基本科研业务费专项资金 (No. 2011JS106) 资助。 |
| 摘 要： | 为了实现硬脂酰-辅酶A脱氢酶1编码基因在乳酸乳球菌中的表达,采用PCR技术扩增获得人类scd1的编码序列。Nco I和Xba I双酶切后定向插入到食品级表达载体pNZ8149中,构建表达载体pNZ8149-scd1。电转化乳酸乳球菌NZ3900,经菌落PCR和测序鉴定scd1基因成功插入到乳酸乳球菌中。在乳链菌肽诱导下进行scd1的表达,转化株提取脂肪酸,进行脂肪酸含量的气相色谱分析。结果显示,SCD1转化菌株中的C16∶1n-7和C18∶1n-7脂肪酸组分比转化pNZ8149的对照组乳酸菌分别提高了92%~169%和53%~127%。文中以scd1基因为例,尝试并证明了脂肪酸脱氢酶类基因能够在食品级乳酸菌中有效表达,为后续研究奠定了基础。 |
| 关 键 词： | 硬脂酰-CoA脱氢酶 乳酸乳球菌 脂肪酸 |
| 收稿时间： | 2012-02-20 |
Heterologous expression of stearoyl-CoA desaturase-1 in Lactococcus lactis NZ3900 |
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| Lamei Wang,Shili Li,Kemian Gou and Yuzhu Luo. Heterologous expression of stearoyl-CoA desaturase-1 in Lactococcus lactis NZ3900[J]. Chinese journal of biotechnology, 2012, 28(9): 1106-1117 | |
| Authors: | Lamei Wang Shili Li Kemian Gou Yuzhu Luo |
| Affiliation: | College Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, Gansu, China; State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China;State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China;State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China;College Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, Gansu, China |
| Abstract: | The possibility of heterologous expression of human Stearoyl-CoA Desaturase(scd1) was investigated.The scd1 encoding sequence was inserted into the pNZ8149 to generate the pNZ8149-scd1 expression plasmids.Then we introduced the pNZ8149-scd1 construct into the Lactococcus lactis NZ3900 to investigate its enzyme activity.The results show that heterologous expressed SCD1 enzyme resulted in a 92%–169% increase in the C16:1n-7 and a 53-127% increase in the C18:1n-7(P<0.05).The SCD1 enzyme was capable of producing n-7 fatty acids in Lactococcus lactis efficiently.It also suggests that the fatty acid desaturases can be heterologous expressed in Lactococcus lactis to produce the helpful fatty acids. |
| Keywords: | stearoyl-CoA desaturase-1 Lactococcus lactis fatty acid |
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