Effect of controlled redox potential and dissolved oxygen on the in vitro refolding of an E. coli alkaline phosphatase and chicken lysozyme |
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Authors: | Angélica Meneses-Acosta Luis Rodolfo Vizcaíno-Meza Hector G Ayala-Castro Martha A Contreras Jaime Ortega-López Octavio T Ramírez |
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Institution: | 1. Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Av. Universidad 1010, Col. Chamilpa, Cuernavaca, Morelos CP 62100, Mexico;2. Departamento de Biotecnología y Bioingeniería, Centro de Investigación y Estudios Avanzados IPN, Av. IPN 2508, Col. San Pedro Zacatenco, México, Distrito Federal, CP 07360, Mexico;3. Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal. 510-3, Cuernavaca, Morelos CP 62250, Mexico |
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Abstract: | The development of efficient purification strategies of recombinant active protein derived from inclusion bodies requires the knowledge of the effect of environmental variables, such as redox potential (RP) and dissolved oxygen tension (DOT), in order to control the protein folding process. However, that information is scarce and only few in vitro studies of the impact of such variables have been reported under constant controlled conditions. In this work, the effect of controlled RP and DOT on the refolding of E. coli alkaline phosphatase (AP) and chicken lysozyme (CL) enzymes were studied. Disulphide bonds of both enzymes were reduced in an instrumented vessel using 2-mercaptoethanol and nitrogen. In the latter case, guanidine hydrochloride was also used to denature the protein. Such conditions caused protein conformational changes, as determined by the intrinsic fluorescence spectra that correlated with a decrease on the activity in both cases. Reduced enzymes were then oxidized, under different constant and predetermined RP or DOT, by manipulating the gas composition in the vessel. Folding kinetics were followed as the recovery of enzyme activity. Results showed that the percentage of recovery and rate of increase of enzymatic activity directly depended on the RP and DOT. A higher folding efficiency was found under controlled DOT compared to controlled RP conditions. These results are useful for establishing protein folding strategies to improve the recovery of active protein from inclusion bodies. |
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Keywords: | Alkaline phosphatase Chicken lysozyme Dissolved oxygen tension Protein refolding Redox potential |
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