Cloning and nucleotide sequence of the bglA gene from Erwinia herbicola and expression of β-glucosidase activity in Escherichia coli |
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Authors: | Laura Marri Silvia Valentini Danielle Venditti |
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Institution: | Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Universitàdi Siena, Via Lalerina 8, 53100 Siena, Italy |
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Abstract: | Abstract Genomic DNA fragments encoding β-glucosidase activity from the wild-type strain WD4 of Erwinia herbicola were cloned into Escherichia coli . Two clones containing a common fragment encoded a polypeptide of 58000 Da. Cloned β-glucosidase, expressed in E. coli , showed activity against natural β-glucoside sugars except for cellobiose. An open reading frame of 1442 bp termed bglA was identified by nucleotide sequencing and it coded for a protein of 480 amino acids ( M r 53896) which showed significant homology with β-glucosidases from glycosyl hydrolase family 1. |
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Keywords: | Erwinia herbicola β-Glucosidase Glycosyl hydrolase family 1 bglA |
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