Triphosphoinositide breakdown and dense body release as the earliest events in thrombin-induced activation of human platelets |
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Authors: | F Rendu P Marche J Maclouf A Girard S Levy-Toledano |
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Institution: | Ajinomoto Co., Inc., Central Research Laboratories 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki-shi, 210 Japan |
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Abstract: | The activation by thrombin of human platelets prelabelled with 32P induced a 30-40% decrease in 32P-triphosphoinositides (TPI) in the first 10 sec; the decrease in the other 32P-labelled phosphoinositides occurred by 20-30 sec. At 10 sec., the intensity of these effects was maximum with 0.2-0.4 U/ml thrombin. Under these conditions, 53, 20 and 15% of the dense granule, alpha-granule and lysosome constituents, respectively were released and thromboxane B2 synthesis reached only 10% of its maximum. Together with experiments carried out with chlorpromazine - or PGE1 - treated platelets, our results suggest the existence of a close relationship between TPI-breakdown and dense body release which appear to be the earliest events resulting from the activation of human platelets by thrombin. |
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Keywords: | PI phosphatidylinositol DPI phosphatidylinositol 4-phosphate or diphosphoinositide TPI phosphatidylinositol 4 5-bisphosphate or triphosphoinositide PA phosphatidic acid β-TG β-thromboglobulin NAG N-acetyl D-glucosaminidase |
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