Folate overproduction in Lactobacillus plantarum WCFS1 causes methotrexate resistance |
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Authors: | Arno Wegkamp Willem M de Vos & Eddy J Smid |
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Institution: | TI Food and Nutrition, Wageningen, The Netherlands;and;NIZO Food Research, Ede, The Netherlands |
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Abstract: | Folate overproduction can serve as a mode of resistance against the folate antagonist methotrexate in Lactobacillus plantarum WCFS1. When compared with a wild-type control strain, an engineered high folate-producing strain was found to be insensitive to methotrexate. The growth rate and the viable count of the folate-overproducing L. plantarum strain were not significantly affected by the presence of methotrexate in the growth medium. |
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Keywords: | folate biosynthesis genes folate overproduction Lactobacillus plantarum methotrexate resistance natural overproduction |
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