Efficient production by Escherichia coli of recombinant protein using salting-out effect protecting against proteolytic degradation |
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| Authors: | Kobayashi Fumihisa Nakamura Yoshitoshi |
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| Affiliation: | (1) Department of Chemistry and Chemical Engineering, Faculty of Engineering, Kanazawa University, 2-40-20 Kodatsuno, Kanazawa, 920-8667, Japan |
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| Abstract: | To produce glucoamylase efficiently as a recombinant protein, E. coli was grown with 20 g (NH4)2SO4 l–1 which removed proteolytic activity but did not effect cell growth. Growth in M9 medium with 20 g (NH4)2SO4 l–1 produced 11 U glucoamylase ml–1 compared to 7 U ml–1 without addition. Furthermore, the glucoamylase activity was maintained at about 9 U ml–1. |
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| Keywords: | glucoamylase proteolytic degradation recombinant Escherichia coli recombinant protein salting-out |
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