人胎脾交错突细胞对T,B淋巴细胞发育影响的免疫组织化学研究

用免疫酶单重和双重染色研究人胎儿脾连续切片中交错突细胞（ＩＤＣ）与Ｔ，Ｂ淋巴细胞的定位关系及ＨＬＡ－ＤＲ表达。结果表明，Ｓ－１００阳性树突状细胞为ＩＤＣ，多数表达ＨＬＡ－ＤＲ。９－１２周的胎脾中就可见到散在分布的ＩＤＣ。１３－１６周胎脾中ＩＤＣ开始定位于白髓的Ｔ细胞集落内和周缘，及Ｂ细胞集落的周边。在上述区域ＩＤＣ常与Ｔ细胞形成ＩＤＣ－Ｔ细胞聚合体。在脾的发育过程中，ＩＤＣ不仅与Ｔ，Ｂ淋巴细胞在分布上关系密切，而且可与这两类细胞形成突起－胞体、胞体－胞体的连接。提示，胎儿脾中ＩＤＣ与Ｔ，Ｂ细胞的迁移，定位及功能成熟过程有密切联系。

IMMUNOHISTOCHEMICAL STUDY OF INFLUENCE OF INTERDIGITATING CELLS(IDCs) ON T.B LYMPHOCYTE DEVELOPMENT IN FETAL SPLEEN

The localization and HLA DR expression of interdigitating cells (IDCs) and T.B lymphocytes were studied by immunoenzyme double staining with S 100, CD3, CD20 and HLA DR antibodies in fetal splecn. The result indicated that S 100 positive dendritic cells were interdigitating cells(IDCs) and most of them were HLA DR positive. At 9-12 weeks of gestation, only a small number of IDCs distributed scatteringly with T lymphocytes and at the periphery of the B cell colony in the spleen. After 13-16 weeks. IDCs began to gather in and around T cell colony in white pulp. The IDCs also distributed around B cell colony and formed contacts with B lymphocytes. In these areas, IDCs and T lymphocytes might form IDC T cell complexes. The structure of IDC T cell complexes and the distribution of IDC were closely related to the migration. residence and maturation of T,B cells in fetal spleen.