Molecular cloning of the RAD10 gene of Saccharomyces cerevisiae |
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| Authors: | L Prakash D Dumais R Polakowska G Perozzi S Prakash |
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| Affiliation: | 2. Department of Radiation Biology and Biophysics, University of Rochester School of Medicine, Rochester, NY 14642 U.S.A. Tel. (716) 275-2656;1. Department of Biology, University of Rochester, Rochester, NY 14627 U.S.A. Tel. (716) 275-3847 |
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| Abstract: | We have cloned the RAD10 gene of Saccharomyces cerevisiae and physically mapped it to a 1.0-kb DNA fragment. Strains containing disruptions of the RAD10 gene were found to show enhanced UV sensitivity compared with the previously characterized rad10-1 or rad10-2 mutants. The UV sensitivity of the disruption mutant is comparable to the highly UV sensitive rad1-19, rad2-delta, and rad3-2 mutants. |
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| Keywords: | Recombinant DNA yeast DNA repair radiation sensitivity M13 phage vector pyrimidine dimers hypersensitivity Δ, deletion kb, kilobases or kilobase pair tet, tetracycline UV, ultraviolet light [], indicates plasmid-carrier state |
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