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Screening of optimal cellulases from symbiotic protists of termites through expression in the secretory pathway of Saccharomyces cerevisiae
Authors:Todaka Nemuri  Nakamura Risa  Moriya Sigeharu  Ohkuma Moriya  Kudo Toshiaki  Takahashi Haruo  Ishida Nobuhiro
Institution:Biotechnology Laboratory, Toyota Central R&D Labs, Inc, Aichi, Japan.
Abstract:For direct and efficient ethanol production from cellulosic materials, we screened optimal cellulases from symbiotic protists of termites through heterologous expression with Saccharomyces cerevisiae. 11 cellulases, belonging to glycoside hydrolase families 5, 7, and 45 endoglucanases (EGs), were confirmed to produce with S. cerevisiae for the first time. A recombinant yeast expressing SM2042B24 EG I was more efficient at degrading carboxylmethyl cellulose than was Trichoderma reesei EG I, a major EG with high cellulolytic activity.
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