In silico feedback for in vivo regulation of a gene expression circuit |
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Authors: | Milias-Argeitis Andreas Summers Sean Stewart-Ornstein Jacob Zuleta Ignacio Pincus David El-Samad Hana Khammash Mustafa Lygeros John |
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Affiliation: | Department of Electrical Engineering, ETH Zurich, Automatic Control Laboratory, Zurich, Switzerland. |
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Abstract: | We show that difficulties in regulating cellular behavior with synthetic biological circuits may be circumvented using in silico feedback control. By tracking a circuit's output in Saccharomyces cerevisiae in real time, we precisely control its behavior using an in silico feedback algorithm to compute regulatory inputs implemented through a genetically encoded light-responsive module. Moving control functions outside the cell should enable more sophisticated manipulation of cellular processes whenever real-time measurements of cellular variables are possible. |
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