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A new insect cell line from the pupal ovary of the Asian corn borer moth Ostrinia furnacalis |
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| Authors: | Ning Zhang Qilian Qin Huan Gong Qian Meng Wei Zhu Menglong Wang Jihong Zhang Guiling Zhou Xuan Li Huan Zhang |
| Institution: | 1. State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences (CAS), Beichenxilu 1-5, Beijing, 100101, China 2. Graduate University of CAS, Beijing, 100049, China 3. School of Biology and Chemical Engineering, Jiangsu University of Science and Technology, Jiangsu, 212018, China |
| Abstract: | Ovaries were removed from Ostrinia furnacalis (Guenée) pupae and were placed in a flask containing TNM-FH medium with 10% inactivated fetal bovine serum. Cell migration occurred after about 1 wk of the initiation in June 2011. The migrated cells were distributed over most of the flask and were treated with N-methyl-N′-nitro-N-nitrosoguanidine (MNNG), a chemical carcinogen, after about 1 mo of initiation for 26 d. Cells were first subcultured successfully 12 d after the MNNG was removed, followed by subculturing for 30 passages. The established cell line, designated IOZCAS-Osfu-1, were analyzed by DNA fingerprinting–PCR (DAF-PCR) to confirm that it originated from O. furnacalis. |
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