显微多道分光光度法研究受柠檬醛损伤后黄曲霉细胞形态变化

采用从山苍籽油分离出的柠檬醛作为抗菌药物，产毒和无毒的黄曲霉细胞（Aspergillus flavus cell, AFC）作为药物作用的靶，以显微多道分光光度法和显微图像分析法对被该醛所损伤的AFC进行图像捕获，测定受损伤后细胞内吸收光谱、截面积、周长、长轴长及短轴长所发生的变化。发现在410nm和665nm处产毒AFC存在特征吸收峰，受该醛损伤后产毒和无毒AFC的吸收光谱波峰均发生迁移，且峰面积增大；截面积等4类形态参数的数值随柠檬醛浓度升高而减少；为质膜物理化学及细胞内生理生化指标变化提供了理论依据。表明柠檬醛不仅破坏质膜的选择性通透性，而且使细胞质膜结构改变并进入细胞，与靶分子及靶细胞器作用而引发一系列新的生理生化现象的出现。实现对活态细胞受药物作用后形态及生物大分子动态变化的快速、实时、在位的测定，对抗菌药物作用于细胞并使其发生形态结构及靶分子的变化提供了必要的物理参数，在药物抗菌理论及方法研究上具有重要意义。

Study on Morphological Change of Aspergillus flavus Damaged by Citral with a Multi-channel Micro-spectrophotometer System

Citral extracted from litsea cubeb oil was used as antibacterial drug; toxigenic and atoxigenic Aspergillus flavus cell (AFC) were used as its target. Multi-channel micro-spectrophotometer (MMSP) and micro-image analysis were applied. Under the pressure of citral, changes in AFC were measured, including items such as absorbance, area, perimeter, major axis length and minor axis length. We found that toxigenic AFC had two characteristic absorbent peaks at 410 nm and 665 nm, and that the waveforms of absorption spectrum of toxigenic and atoxigenic AFC migrated and their peak areas increased, and that four morphological parameters reduced with citral concentration increasing. The results suggest that citral damages selective permeability and structure of membrane to enter to cell, and acts on target molecule or organelle to bring changes in physiology and biochemistry. In real-time, in-situ and non-invasive situation, the dynamic changes in shape and intracellular macromolecule are fastly measured during the drugs damage a living cell, which not only provides these changes with necessary physical parameters, but also has an importance in theoretical research and experimental method