A method for analysis of chromosomes in hybrid cells employing sequential G-banding and mouse specific C-banding

A sequential banding technique is described for the identification of chromosomes of interspecific hybrid cells with a mouse parent. Metaphases were first G-banded using trypsin-Giemsa to identify individual chromosomes and then the centromeres of the same cells were differentially stained by a C-banding technique specific for mouse chromosomes. This mouse specific C-banding employs treatment with hot formamide-SSC before staining, and the effect of this treatment on the staining of chromosomes from a number of species was investigated. The specific staining of mouse centromeres confirms the parental origin of chromosomes identified by G-banding and allows the rapid recognition of mouse and non-mouse chromosomes in metaphases from many different hybrid combinations.