Expression of microtubule-associated protein TPX2 in human gastric carcinoma and its prognostic significance |
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Authors: | Cuijie Shao Changsheng Duan Jiani Wang Shunlian Luan Yong Gao Dan Jin Deqiang Wang Yuming Li Lihua Xu |
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Institution: | 1.Department of Pain,Affiliated Hospital of Binzhou Medical College,Binzhou,China;2.Department of Gastrointestinal Surgery,Affiliated Hospital of Binzhou Medical College,Binzhou,China;3.Breast Cancer Center,The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou,China;4.Department of Oncology,Affiliated Hospital of Binzhou Medical College,Binzhou,China;5.Department of Hematology,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou,China;6.Guangdong Key Laboratory of Urology,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou,China |
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Abstract: | BackgroundThe molecular mechanisms underlying the development and progression of gastric carcinoma remain poorly understood. The main objective of this study was to investigate the expression level of targeting protein for Xenopus kinesin-like protein 2 (TPX2) and its clinical significance in human gastric carcinoma.MethodsReal-time quantitative polymerase chain reaction (RT-PCR) and western blotting were used to determine the mRNA and protein levels of TPX2 in 20 paired gastric carcinoma tissues and the adjacent normal tissues, and the expression of TPX2 protein in 106 specimens of a gastric carcinoma tissue microarray was determined by immunohistochemistry. The associations of TPX2 expression with the clinicopathological features were analyzed, and the prognosis of gastric carcinoma patients was evaluated.ResultsThe results showed that the expression of TPX2 mRNA was significantly higher in gastric carcinoma than in the adjacent normal tissues in 20 paired samples. Western blotting analysis revealed that TPX2 protein was differentially increased in 17 of 20 specimens from primary human gastric carcinoma tissues compared with those from adjacent non-tumor tissues. Immunohistochemical staining showed that TPX2 over-expression was significantly associated with advanced age (P = 0.001) and tumor T stage (P = 0.003). In addition, TPX2 was an independent prognostic factor for overall survival (OS) in the multivariate analysis hazard ratio (HR) 0.001; 95 % confidence interval (CI) 2.626–7.198; P = 0.001].ConclusionsTPX2 is up-regulated in gastric carcinoma and is associated with old age and tumor T stage. TPX2 may serve as a good prognostic indicator in patients with gastric carcinoma. |
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