Determination of E. gracilis mRNA base composition using high-pressure liquid chromatography. |
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| Authors: | K H Falchuk C Hardy |
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| Affiliation: | 1. Biophysies Research Laboratory, Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts, USA;2. Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA;3. the Division of Medical Biology, Peter Bent Brigham Hospital, Boston, Massachusetts, USA |
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| Abstract: | The purine and pyrimidine bases of E. gracilis have been separated using a high-pressure liquid chromatograph (HPLC). Each base was unambiguously identified by its characteristic elution profile and UV absorption spectrum. This method allows for the study of the base composition of mRNAs altered by pathological processes, exemplified here by the analysis and comparison of mRNAs from zinc sufficient and deficient organisms. |
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