Bradykinin Activates a Phospholipase D that Hydrolyzes Phosphatidylcholine in PC12 Cells |
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Authors: | Joel Horwitz |
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Institution: | Department of Pediatrics, University of Chicago, IL 60637. |
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Abstract: | In PC12 pheochromocytoma cells whose phospholipids had been prelabelled with 3H]palmitic acid, bradykinin increased the production of 3H]phosphatidic acid. The increase in 3H]phosphatidic acid occurred within 1-2 min. before the majority of the increase in 3H]diacylglycerol. When the phospholipids were prelabeled with 3H]choline, bradykinin increased the intracellular release of 3H]choline. The production of phosphatidic acid and choline suggests that bradykinin was increasing the activity of phospholipase D. Transphosphatidylation is a unique property of phospholipase D. In cells labeled with 3H]palmitic acid, bradykinin stimulated the transfer of phosphatidyl groups to both ethanol and propanol to form 3H]phosphatidylethanol and 3H]phosphatidylpropanol, respectively. The effect of bradykinin on 3H]phosphatidic acid and 3H]phosphatidylethanol formation was partially dependent on extracellular Ca2+. In cells treated with nerve growth factor, carbachol also increased 3H]phosphatidylethanol formation. To investigate the substrate specificity of phospholipase D, cells were labeled with 14C]stearic acid and 3H]palmitic acid, and then incubated with ethanol in the absence or presence of bradykinin. The 14C/3H ratio of the phosphatidylethanol that accumulated in response to bradykinin was almost identical to the 14C/3H ratio of phosphatidylcholine. The 14C/3H ratio in phosphatidic acid and diacylglycerol was higher than the ratio in phosphatidylcholine. These data provide additional support for the idea that bradykinin activates a phospholipase D that is active against phosphatidylcholine. The hydrolysis of phosphatidylcholine by phospholipase D accounts for only a portion of the phosphatidic acid and diacylglycerol that accumulates in bradykinin-stimulated cells: bradykinin evidently stimulates several pathways of phospholipid metabolism in PC12 cells. |
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Keywords: | Phospholipase D Phosphatidic acid Phosphatidylethanol Choline Carbachol Bradykinin PC12 cells |
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