Enhanced ascorbic acid accumulation in transgenic potato confers tolerance to various abiotic stresses |
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Authors: | Hemavathi Chandrama Prakash Upadhyaya Nookaraju Akula Ko Eun Young Se Chul Chun Doo Hwan Kim and Se Won Park |
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Institution: | (1) Department of Molecular Biotechnology, Konkuk University, Seoul, South Korea; |
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Abstract: | l-Ascorbic acid (Vitamin C, AsA) is an important component of human nutrition. Plants and several animals can synthesize their
own ascorbic acid, whereas humans lack the gene essential for ascorbic acid biosynthesis and must acquire from their diet.
In the present study, we developed transgenic potato (Solanum tuberosum L. cv. Taedong Valley) over-expressing l-gulono-γ-lactone oxidase (GLOase gene; NCBI Acc. No. NM022220), isolated from rat cells driven by CaMV35S constitutive promoter
that showed enhanced AsA accumulation. Molecular analyses of four independent transgenic lines performed by PCR, Southern
and RT-PCR revealed the stable integration of the transgene in the progeny. The transformation frequency was ca. 7.5% and
the time required for the generation of transgenic plants was 6–7 weeks. Transgenic tubers showed significantly enhanced AsA
content (141%) and GLOase activity as compared to untransformed tubers. These transgenics were also found to withstand various
abiotic stresses caused by Methyl Viologen (MV), NaCl or mannitol, respectively. The T1 transgenic plants exposed to salt stress (100 mM NaCl) survived better with increased shoot and root length when compared
to untransformed plants. The elevated level of AsA accumulation in transgenics was directly correlated with their ability
to withstand abiotic stresses. These results further demonstrated that the overexpression of GLOase gene enhanced basal levels of AsA in potato tubers and also the transgenics showed better survival under various abiotic
stresses. |
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