Chitinase induction in onion tissue cultures |
| |
| Authors: | Kevin J Williams David W M Leung |
| |
| Institution: | (1) Department of Plant and Microbial Sciences, University of Canterbury, Christchurch, New Zealand;(2) Present address: Department of Crop Protection, University of Adelaide, Waite Campus, 5064 Glen Osmond, South Australia |
| |
| Abstract: | Chitinase activity in Allium cepa (onion) tissue cultures was investigated using a radioactive assay and polyacrylamide gel electrophoresis (PAGE). Increased chitinase activity was detected in callus cultured on agar-solidified media for 72 h with 1 mM salicylic acid or 0.05 M ethrel, and in callus cultured in liquid suspension for 72 h with 0.1 mM salicylic acid or 0.1 M ethrel. Non-denaturing PAGE analysis of callus suspension cultures treated with salicylic acid revealed a new chitinase activity which accumulated in the fluid of the suspension cultures. |
| |
| Keywords: | Allium cepa callus ethylene PAGE salycilic acid suspension culture |
| 本文献已被 SpringerLink 等数据库收录！ |
