In vitro organogenesis from diploid tissues of Cycas revoluta Thunb.

Leaf base and mesocotyl explants derived from in vitro-grown seedlings of Echinochloa colona were cultured on Murashige and Skoog's (MS) medium containing various concentrations of benzyladenine (BA), -naphthaleneacetic acid (NAA) and kinetin. Leaf base and mesocotyl segments exhibited optimal morphogenetic response by using 6.66 M BA with 2.68 M NAA. Induction of rooting from regenerated shoots was readily achieved in half strength MS medium without organics and growth regulators. Histological studies revealed the sequence of shoot bud regeneration in the monocot system. The in vitro-raised plants were established in chromite minewaste.Abbreviations BA benzyladenine - NAA -naphthaleneacetic acid - MS medium Murashige & Skoog's (1962) medium