A simple and sensitive fluorometric assay for tyrosine hydroxylase. |
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Authors: | T Yamauchi H Fujisawa |
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Institution: | Department of Biochemistry, Asahikawa Medical College, Asahikawa 078-11, Japan |
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Abstract: | A simple, rapid, and sensitive fluorometric assay for measuring the activity of tyrosine hydroxylase is described. The enzyme activity is detected by converting tyrosine to 3,4-dihydroxyphenylalanine (dopa), which is then subjected to conversion to the highly fluorescent product by the trihydroxyindole method. The assay method is very reproducible, more sensitive than a radiochemical method for the determination of tyrosine hydroxylase activity using the isolation of 3H]water commonly used, and linear from 0.2 to 12 nmol of dopa. The method should be applicable for the assay of the enzyme with a wide range of activity. |
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