Changes in lectin-induced deoxyribonucleic acid synthesis in cultures of chick-embryo fibroblasts at various stages of development |
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| Authors: | Roland Roguet and Roland Bourrillon |
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| Institution: | Laboratoire de Biochimie, Centre de Recherches sur les Protéines, Faculté de Médecine Lariboisière, Saint Louis (Université Paris VII), 45 Rue des Saints Pères, 75006 Paris, France |
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| Abstract: | Concanavalin A and Robinia pseudoacacia lectin decreased (3)H]thymidine incorporation into acid-insoluble material of fibroblasts cultured from 6-10-day chick embryos. In contrast, these lectins stimulated (3)H]thymidine incorporation in cells from 16-day embryos. These effects are due to neither (3)H]thymidine permeability modification nor toxicity of the lectins. The specificity of lectin action was proved by blocking experiments with alpha-methyl mannopyranoside and with anti-(Robinia lectin) serum. |
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