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Antifungal Activity and Action Mechanism Study of Coumarins from Cnidium monnieri Fruit and Structurally Related Compounds |
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| Authors: | Ying-Hui He Xiao-Fei Shang Hai-Xin Li An-Ping Li Chen Tang Bao-Qi Zhang Zhi-Jun Zhang Rui Wang Yue Ma Sha-Sha Du Yong-Mei Hu Tian-Lin Wu Wen-Bin Zhao Cheng-Jie Yang Ying-Qian Liu |
| Institution: | 1. School of Pharmacy, Lanzhou University, Lanzhou, 730000 P. R. China
State Key Laboratory of Grassland Agro-ecosystems, Lanzhou University, Lanzhou, 730000 China These authors contributed equally to this work.;2. School of Pharmacy, Lanzhou University, Lanzhou, 730000 P. R. China These authors contributed equally to this work.;3. School of Pharmacy, Lanzhou University, Lanzhou, 730000 P. R. China;4. CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, 730000 P. R. China Gansu Institute for Drug Control, Lanzhou, 730000 P. R. China;5. Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong Province, Weifang University, Weifang, 261061 China. |
| Abstract: | The increasing resistance of plant diseases caused by phytopathogenic fungi highlights the need for highly effective and environmentally benign agents. The antifungal activities of Cnidium monnieri fruit extracts and five isolated compounds as well as structurally related coumarins against five plant pathogenic fungi were evaluated. The acetone extract, which contained the highest amount of five coumarins, showed strongest antifungal activity. Among the coumarin compounds, we found that 4-methoxycoumarin exhibited stronger and broader antifungal activity against five phytopathogenic fungi, and was more potent than osthol. Especially, it could significantly inhibit the growth of Rhizoctonia solani mycelium with an EC50 value of 21 μg mL?1. Further studies showed that 4-methoxycoumarin affected the structure and function of peroxisomes, inhibited the β-oxidation of fatty acids, decreased the production of ATP and acetyl coenzyme A, and then accumulated ROS by damaging MMP and the mitochondrial function to cause the cell death of R. solani mycelia. 4-Methoxycoumarin presented antifungal efficacy in a concentration- dependent manner in vivo and could be used to prevent the potato black scurf. This study laid the foundation for the future development of 4-methoxycournamin as an alternative and friendly biofungicide. |
| Keywords: | Cnidium monnieri coumarins antifungal activity 4-methoxycoumarin |