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Chemical Constituents from Staminate Flowers of Eucommia ulmoides Oliver and Their Anti-Inflammation Activity in Vitro |
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| Authors: | Rui Li Tao Huang Lanlan Nie An Jia Lei Zhang Ying Yuan Yanlong Hong Jianying Wang Xiao Hu |
| Affiliation: | 1. Shanghai Innovation Center of Health Service, Shanghai University of Traditional Chinese Medicine, No. 1220, Cailun Rd., Shanghai, 201203 P. R. China Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, 285 Gebaini Road, Shanghai, 201203 P. R. China These authors contributed equally to this work.;2. Medical School, Huanghe Science and Technology College, Zhengzhou, 450000 P. R. China These authors contributed equally to this work.;3. Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, 285 Gebaini Road, Shanghai, 201203 P. R. China;4. Medical School, Huanghe Science and Technology College, Zhengzhou, 450000 P. R. China;5. Shanghai Innovation Center of Health Service, Shanghai University of Traditional Chinese Medicine, No. 1220, Cailun Rd., Shanghai, 201203 P. R. China |
| Abstract: | Three new monoterpenoids, named eucomylides A−C ( 1 – 3 ), along with six known compounds ( 4 – 9 ) were isolated from the staminate flowers of Eucommia ulmoides Oliver. The structures were elucidated by extensive analyses of spectroscopic methods, and their absolute configurations were established by time-dependent density functional theory electronic circular dichroism (TDDFT ECD) calculation. All the compounds along with previously isolated components ( 10 – 14 ) were tested for their anti-inflammatory effects. Two iridoid glycosides ( 11 and 12 ) and a flavonoid glycoside ( 14 ) showed potent suppressive effects on nitric oxide (NO) production in RAW 264.7 cells, with IC50 values ranging from 17.11 to 22.26 μM. |
| Keywords: | Eucommia ulmoides Oliver Eucommiaceae eucomylides A−C monoterpenoid anti-inflammatory activity |