Enhanced Wound Healing Activity of Undenatured Type I Collagen Isolated from Discarded Skin of Black Sea Gilthead Bream (Sparus aurata) Conditioned as 3D Porous Dressing |
| |
| Authors: | Alexandra Gaspar-Pintiliescu Elena Daniela Anton Andreea Iosageanu Daniela Berger Cristian Matei Raul-Augustin Mitran Ticuta Negreanu-Pirjol Oana Craciunescu Lucia Moldovan |
| |
| Institution: | 1. Department of Cellular and Molecular Biology, National Institute of R&D for Biological Sciences, 296, Bucureşti, Splaiul Independentei, 060031 Bucharest, Romania;2. Faculty of Applied Chemistry and Material Science, University ‘Politehnica' of Bucharest, 1–7, Bucureşti, Polizu Street, 011061 Bucharest, Romania;3. Department of Oxide Compounds and Materials Science, ‘Ilie Murgulescu' Institute of Physical Chemistry, Romanian Academy, 202, Bucureşti, Splaiul Independentei, 060021 Bucharest, Romania;4. Faculty of Pharmacy, Ovidius University of Constanta, 1, University Alley, 900470 Constanta, Romania |
| |
| Abstract: | Acid-soluble, undenatured, type I collagen (BSC) isolated, for the first time, from gilthead bream skin and the novel fabricated 3D porous wound dressing were analyzed for physicochemical and biological properties, in order to offer a safe alternative to commercial bovine collagen (BC) products. SDS-polyacrylamide analysis confirmed the purity of BSC preparation. The hydroxyproline content and temperature of denaturation of BSC were lower than those of BC, in accordance with the structural data recorded by FT-IR spectroscopy. However, certain concentrations of BSC stimulated the cell metabolism of L929 fibroblasts in a higher proportion than BC. The 3D wound dressing presented high porosity and low surface hydrophobicity that could help cell attachment and growth. The rapid biodegradation of BSC wound dressing could explain the improved in vitro cell migration and wound closure rate. In conclusion, the skin of gilthead bream from the Black Sea coast represented a valuable source for the biomedical industry, providing biocompatible, biodegradable collagen and 3D porous wound dressing, as novel material with enhanced wound healing activity. |
| |
| Keywords: | biomaterials proteins biological activity marine collagen wound healing |
|
|
