| Affiliation: | 1. Department of Pharmacy, Pharmacognosy Laboratory, Federal University of Rio Grande do Sul, Porto Alegre, 90610-000 Brazil;2. Department of Pharmacy, Pharmacognosy Laboratory, Federal University of Rio Grande do Sul, Porto Alegre, 90610-000 Brazil Postgraduate Program in Pharmaceutical Sciences, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, 90610-000 Brazil;3. Keizo-Asami Immunopathology Laboratory, Federal University of Pernambuco, Recife, Pernambuco, 50670-901 Brazil;4. Department of Environmental Impact Assessment, Unilasalle, Canoas, Rio Grande do Sul, 92010-000 Brazil;5. Postgraduate Program in Pharmaceutical Sciences, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, 90610-000 Brazil |
| Abstract: | The present study investigates the chemical composition, anti-inflammatory, and antihypertensive activities, in vitro, from extracts of Cuphea lindmaniana and Cuphea urbaniana leaves. The extraction was performed ultrasound-assisted, and UHPLC/MS analysis was in positive mode ionization. The anti-inflammatory activity of the extracts and miquelianin were assayed at concentrations 0.001–10 μg/mL by chemotaxis on rat polymorphonuclear neutrophils. The antihypertensive activity was performed by angiotensin-converting enzyme (ACE) inhibition. From the nineteen proposed compounds, six of them are described for the first time in this genus. The extracts displayed antichemotactic effect with a reduction of 100 % of the neutrophil migration, in vitro, in most concentrations. The ACE-inhibition presented results ranging from 19.58 to 22.82 %. In conclusion, C. lindmaniana and C. urbaniana extracts contain a rich diversity of flavonoids and display in vitro anti-inflammatory and antihypertensive potential. Thus, this study could serve as a scientific baseline for further investigation, on developmental novel products with therapeutic actions. |
