Recombineering facilitates the discovery of natural product biosynthetic pathways in Pseudomonas parafulva |
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| Authors: | Wentao Zheng Xue Wang Yuwei Chen Yachao Dong Diao Zhou Ruxin Liu Haibo Zhou Xiaoying Bian Hailong Wang Qiang Tu Vinothkannan Ravichandran Youming Zhang Aiying Li Jun Fu Jia Yin |
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| Affiliation: | 1. Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, People's Republic of China;2. Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China |
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| Abstract: | Microbial natural products among other functions they play a vital role in the disease prevention in humans, animals and plants. Pseudomonas parafulva CRS01-1 is a broad-spectrum antagonistic bacterium present in plants. However, no natural products have been isolated from this strain till date. Corresponding biosynthetic gene clusters to natural products is an effective method for bioprospecting, for which, genome manipulation tools are essential. We previously developed Pseudomonas-specific phage-derived homologous recombination systems for genetic engineering in four Pseudomonas species. Herein, we report the application of these recombineering systems in Pseudomonas parafulva CRS01-1, along with structural elucidation and bioactivity evaluation of natural products. The Pseudomonas recombineering toolbox established before in different four species is efficient for genome mining and bioactive metabolite discovery from more distant species. |
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| Keywords: | biosynthetic gene clusters, genome mining Pseudomonas parafulva, recombineering |
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