Metabolic Profiling of Vasorelaxant Extract from Malvaviscus arboreus by LC/QTOF-MS |
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| Authors: | Sergio Rodríguez-Morales Blanca Ocampo-Medina Nancy Romero-Ceronio Cuauhtémoc Alvarado-Sánchez Miguel Ángel Vilchis-Reyes Luis Fernando Roa de la Fuente Rolffy Ortiz-Andrade Oswaldo Hernández-Abreu |
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| Affiliation: | 1. Unidad de Química-Sisal, Facultad de Química, Universidad Nacional Autónoma de México, Puerto de abrigo S/N, 97356 Sisal, Yucatán, Mexico;2. Centro de Investigación de Ciencia y Tecnología Aplicada de Tabasco, Universidad Juárez Autónoma de Tabasco, Carretera Cunduacán-Jalpa km. 1, Cunduacán, 86690 Tabasco, Mexico;3. Facultad de Química, Universidad Autónoma de Yucatán, Mérida, Calle 43 N. 613, 97069 Mérida, Yucatán, Mexico |
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| Abstract: | We aimed to develop a standardized methodology to determine the metabolic profile of organic extracts from Malvaviscus arboreus Cav. (Malvaceae), a Mexican plant used in traditional medicine for the treatment of hypertension and other illnesses. Also, we determined the vasorelaxant activity of these extracts by ex vivo rat thoracic aorta assay. Organic extracts of stems and leaves were prepared by a comprehensive maceration process. The vasorelaxant activity was determined by measuring the relaxant capability of the extract to decrease a contraction induced by noradrenaline (0.1 μM). The hexane extract induced a significant vasorelaxant effect in a concentration- and endothelium-dependent manner. Secondary metabolites, such as polyunsaturated fatty acids, terpenes and one flavonoid, were annotated by liquid chromatography/quadrupole time-of-flight mass spectrometry (LC/QTOF-MS) in positive ion mode. This exploratory study allowed us to identify bioactive secondary metabolites from Malvaviscus arboreus, as well as identify potentially-new vasorelaxant molecules and scaffolds for drug discovery. |
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| Keywords: | bioactive LC/QTOF-MS Malvaviscus arboreus metabolic profiling pharmacognosy |
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