Sporulation: an alternative way to recover recombinant proteins from Bacillus subtilis |
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Authors: | Maurizi G Bossù P Boraschi D Ulisse E Tagliabue A Ruggiero P |
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Affiliation: | Consorzio Biolaq, Via Campo di Pile, I-67100 L'Aquila, Italy. |
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Abstract: | A recombinant strain of Bacillus subtilis engineered for endocellular expression of human interleukin-1 receptor antagonist (IL-Ira) was subjected to sporulation. The recombinant protein was recovered from the sporulation supernatant in quantities, purity, and activity comparable with those obtained from a traditional cell lysate. Thus, exploitation of this natural mechanism of autolysis could overcome problems of intact protein recovery related to the cell disruption step. (c) 1995 John Wiley & Sons, Inc. |
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Keywords: | recombinant protein purification B. subtilis sporulation cell lysis |
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