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Location of the SH group of the alkali light chain on the myosin head as revealed by electron microscopy |
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| Authors: | K Yamamoto M Tokunaga K Sutoh T Wakabayashi T Sekine |
| Affiliation: | 1. Department of Biochemistry Juntendo University, School of Medicine Hongo, Bunkyo-ku, Tokyo 113, Japan;2. Department of Physics, Faculty of Science University of Tokyo, Hongo Bunkyo-ku, Tokyo 113, Japan;3. Department of Biophysics and Biochemistry Faculty of Science, University of Tokyo Hongo, Bunkyo-ku, Tokyo 113, Japan |
| Abstract: | The location of the single cysteinyl residue of the alkali light chain on the myosin head was determined by electron microscopy. The cysteinyl residue of isolated alkali light chain 2 was biotinylated and the light chain was exchanged with that of heavy meromyosin in 4.7 M-NH4Cl. Avidin was attached to the biotin in the heavy meromyosin and the complex was rotary shadowed and observed in the electron microscope. The distance from the head-rod junction to the centre of avidin was 8(+/- 3) nm (mean value +/- standard deviation: n = 105). |
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