| 荸荠皮多糖体外清除自由基活性的研究 |
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| 引用本文： | 李婕姝,贾冬英,姚开,刘祥梅.荸荠皮多糖体外清除自由基活性的研究[J].氨基酸和生物资源,2008,30(4). |
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| 作者姓名： | 李婕姝 贾冬英 姚开 刘祥梅 |
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| 作者单位： | 四川大学轻纺与食品学院,成都,610065;四川大学轻纺与食品学院,成都,610065;四川大学轻纺与食品学院,成都,610065;四川大学轻纺与食品学院,成都,610065 |
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| 摘 要： | 采用DPPH自由基法、邻苯三酚自氧化法和水杨酸法分别表征了荸荠皮多糖清除DPPH自由基(DPPH.)、超氧阴离子自由基(O2-.)和羟基自由基(.OH)的能力。结果显示,脱蛋白和未脱蛋白荸荠皮多糖具有清除自由基的活性,但该活性明显低于茶多酚。脱蛋白多糖清除DPPH.的能力高于未脱蛋白多糖,其清除50%DPPH.的作用质量浓度(EC50)分别为50.26 mg.L-1和76.22 mg.L-1。两种多糖清除.OH和O2-.的能力相当,其EC50(g.L-1)分别为0.118和0.124以及10.87和9.53。
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| 关 键 词： | 荸荠皮 多糖 自由基 清除活性 |
In Vitro Radical-Scavenging Activity of Polysaccharides from Eleocharis tuberosa Peels |
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| LI Jie-shu,JIA Dong-ying,YAO Kai,LIU Xiang-mei.In Vitro Radical-Scavenging Activity of Polysaccharides from Eleocharis tuberosa Peels[J].Amino Acids & Biotic Resources,2008,30(4). |
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| Authors: | LI Jie-shu JIA Dong-ying YAO Kai LIU Xiang-mei |
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| Abstract: | The abilities of polysaccharides from Eleocharis tuberosa peels to scavenge the radicals of 1,1-diphenyl-2-picryl hydrazyl(DPPH·),superoxide anion(O2-·) and hydroxyl(O2-·) were studied using the methods of DPPH radical,pyrogallol autoxidation and salicylic acid,respectively.The results showed that the polysaccharides possessed a radical-scavenging activity,which was much lower than that of tea polyphenols.Polysaccharides without proteins demonstrated a higher ability to scavenge DPPH· than polysaccharides with proteins,their concentrations to eliminate 50% DPPH radicals(EC50,μg/ml) being 0.26 and 76.22,respectively.They exhibited a similar ability to scavenge ·OH and O2-·,their EC50(mg/ml) being 0.118,0.124,10.87 and 9.53,respectively. |
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| Keywords: | Eleocharis tuberosa peels polysaccharides free radicals radical-scavenging activity |
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