Activation of phospholipase D induced by hydrogen peroxide in suspension-cultured rice cells |
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Authors: | Yamaguchi Takeshi Tanabe Shigeru Minami Eiichi Shibuya Naoto |
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Institution: | Department of Rice Research, National Agricultural Research Center, National Agricultural and Bio-oriented Research Organization, Joetsu, Niigata, 943-0193 Japan. tkyama@affrc.go.jp |
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Abstract: | Hydrogen peroxide (H2O2) (10-100 microM) induced rapid and transient accumulation of phosphatidic acid (PA) in suspension-cultured rice cells. When phospholipase activity in the cellular extract fraction prepared from rice cells treated with H2O2 was assayed in the presence of 1-butanol (0.1%), rapid and transient phosphatidylbutanol (PtdBut) formation was observed. Thus, the H2O2-activated phospholipase was concluded to be phospholipase D (PLD). Furthermore, H2O2 directly induced in vitro PLD activation in the cytosolic fraction without H2O2 treatment. In vitro and in vivo activation of PLD were completely suppressed in the presence of lavendustin A (0.05 mM), a potent inhibitor of protein tyrosine kinase. Phytoalexin biosynthesis induced by N-acetylchitooligosaccharide elicitor was enhanced in the presence of H2O2 (10-100 microM), whereas it was suppressed in the presence of tiron, a potent scavenger of O2-, 1-butanol (0.1%) and lavendustin A (0.05 mM). These results indicate that H2O2-inducible PLD activation enhances signal transduction leading to phytoalexin biosynthesis in rice cells. |
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