Differential Responses in Activity of Antioxidant Enzymes to Different Environmental Stresses in Arabidopsis thaliana |
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Authors: | Akihiro Kubo Mitsuko Aono Nobuyoshi Nakajima Hikaru Saji Kiyoshi Tanaka Noriaki Kondo |
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Institution: | (1) Environmental Biology Division, National Institute for Environmental Studies, 16–2 Onogawa, Tsukuba, Ibaraki, 305–0053 Japan, JP;(2) Regional Environment Division, National Institute for Environmental Studies, 16–2 Onogawa, Tsukuba, Ibaraki, 305–0053 Japan, JP;(3) Faculty of Agriculture, Tottori University, Koyama, Tottori, 680–0945 Japan, JP;(4) Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, 113–0033 Japan, JP |
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Abstract: | Arabidopsis thaliana . Three-week-old plants were exposed to a high temperature (30 C), an enhanced light intensity (200 μE/m2/sec), water deficiency (water deprivation for 2 days), a chilling temperature (5 C), or ultraviolet-B (UV-B) radiation (0.25
or 0.094 W/m2) for 1 week (except for water deficiency). The high temperature and enhanced light treatments increased only dehydroascorbate
reductase (DHAR) activity. Water deficiency enhanced the activities of DHAR and guaiacol peroxidase (PER). Chilling temperature
increased the activities of ascorbate peroxidase (APX) and glutathione reductase (GR), whereas it decreased catalase (CAT)
activity. UV-B at an intensity of 0.25 W/m2 elevated the activities of APX, monodehydroascorbate reductase (MDHAR), GR, PER and superoxide dismutase (SOD). It was suggested
that the amounts of phenylpropanoid compounds increased during treatments of plants with enhanced light intensity, chilling
temperature, and UV-B. These results suggest that some differences exist among the oxidative stress conditions caused by the
different treatments, although all of these treatments seem to be related to active oxygen production. We propose that in
A. thaliana, environmental stresses may be classified into those which induce DHAR activity and those which induce APX activity.
Received 11 January 1999/ Accepted in revised form 22 April 1999 |
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Keywords: | : Active oxygen Antioxidant enzyme Arabidopsis thaliana Environmental stress Enzymatic activity Phenylpropanoid |
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