Pararosaniline Or Acriflavine-Schiff Staining of Epoxy Embedded Tissue After Periodic Acid Oxidation in Ethanol: A Method Suitable for Morphometric and Fluorometric Analysis of Glycogen |
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| Authors: | Laurence Ferey Paulette Herlin Jacques Marnay Anne-marie Mandard Robert Catania Pierre Lubet Richard Lande Daniel Bloyet |
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| Affiliation: | a Pathologic Anatomy Seruices, Franpis Baclesse Regional Center and University Hospital Center and Uniuersity Center for Biomedical Image Analysis, Caen, France |
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| Abstract: | A method for preparing tissue sections for automatic image analysis of glyco-gen is described. Large semithin seaions of epoxy embedded tissue fixed in glutaraldehyde osmium were stained with Schiff reagent and acriflavine (fluorescent staining) after resin removal and periodic acid oxidation in ethanol. We found it essential to avoid tissue rehydra-tion before final staining. The Schiff stain permits an assessment of the cellular volume of glycogen, and the acriflavine allows a fluorometric evaluation of glycogen density. |
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