Glucose regulated proteins 78 and 75 bind to the receptor for hyaluronan mediated motility in interphase microtubules |
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Authors: | Kuwabara Hiroko Yoneda Masahiko Hayasaki Hana Nakamura Toshiya Mori Hiroshi |
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Affiliation: | Department of Pathology, Osaka Medical College, 2-7, Takatsuki, Osaka 569-8686, Japan. pa2020@art.osaka-med.ac.jp |
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Abstract: | The receptor for hyaluronan mediated motility (RHAMM), which is a hyaluronan-binding protein, is a centrosomal and microtubal protein. Here, we have identified two RHAMM-binding proteins, glucose regulated protein (GRP) 78 and GRP75, using co-immunoprecipitation analysis. These two proteins directly bound to glutathione-S-transferase-RHAMM fusion proteins. By double immunostaining, GRP78 and GRP75 colocalized with RHAMM in interphase microtubules, but were separated in mitotic spindles. Prevention of microtubule polymerization by TN-16 and vincristine sulfate induced RHAMM overexpression without a significant change in GRP78/75. Taken together, GRP78/75 and RHAMM complexes may stabilize microtubules in the interphase, associated with a downregulation of RHAMM. These results reveal a new biochemical activity of RHAMM. |
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Keywords: | RHAMM GRP78 GRP75 Heat shock protein 70 Lymphoma |
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