| 天蚕抗菌肽Ａ与蜂毒素杂合肽基因的合成及在大肠杆菌中的克隆与表达 |
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| 引用本文： | 朱嘉明,刘飞鹏,李月琴,钟振宇,张娜. 天蚕抗菌肽Ａ与蜂毒素杂合肽基因的合成及在大肠杆菌中的克隆与表达[J]. 遗传, 2002, 24(1): 31-34 |
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| 作者姓名： | 朱嘉明 刘飞鹏 李月琴 钟振宇 张娜 |
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| 作者单位： | 暨南大学生命科学技术学院生物工程学系，广州 510630 Department of Biotechnology,College of Life Scienceand Technology,Jinan University,Guangzhou 510630,China |
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| 基金项目： | 广东省自然科学基金资助 (No .970 6 36 ) |
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| 摘 要： | 根据天蚕抗菌肽Ａ（cecropin A,CA）Ｎ端第１～７个 氨基酸残基、 蜂毒素（melittin,M）Ｎ端第５～１２个氨基酸残基,以大肠杆菌偏爱的密码子设计合成了杂合肽ＣＡ（１～７）-Ｍ（５～1２）基因,同载体pＧＥＭＥＸ-１连接后转化大肠杆菌ＪＭ１０９，经打点杂交筛选和序列测定,获得一个与设计一致的克隆。将此克隆中的质粒亚克隆至JM103(DE3) ，经IPTG诱导、BrCN切割和抑菌圈试验表明，克隆的杂合肽基因在JM109(DE3)中获得了表达。Abstract:According to the studies of David Andreu and other scientists,we designed and synthesized a gene encoding shortened cecropin A-melittin hybrid.This hybrid consists of CA(1-7) and M(5-12).The gene was inserted into pGEMEX-1 in the site of EcoRI and BamHI.A positive clone was obtained by hybridization and DNA sequencing and was expressed in JM109(DE3).
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| 关 键 词： | 天蚕抗菌肽Ａ 蜂毒素 杂合肽
Key words cecropin A melittin hybrid |
| 文章编号： | 0253-9772(2002)01-0031-04 |
| 修稿时间： | 2001-04-06 |
Cloning and Expression of a Gene Encoding Shortened Cecropin A-Melittin Hybrid in E.coli |
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| ZHU Jia-ming,LIU Fei-peng,LI Yue-qing,ZHONG Zhen-yu,ZHANG Na. Cloning and Expression of a Gene Encoding Shortened Cecropin A-Melittin Hybrid in E.coli[J]. Hereditas, 2002, 24(1): 31-34 |
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| Authors: | ZHU Jia-ming LIU Fei-peng LI Yue-qing ZHONG Zhen-yu ZHANG Na |
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| Affiliation: | Department of Biotechnology, College of Life Scienceand Technology, Jinan University, Guangzhou 510630, China. tom@jnu.edu.cn |
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| Abstract: | According to the studies of David Andreu and other scientists,we designed and synthesized a gene encoding shortened cecropin A-melittin hybrid.This hybrid consists of CA(1-7) and M(5-12).The gene was inserted into pGEMEX-1 in the site of EcoRI and BamHI.A positive clone was obtained by hybridization and DNA sequencing and was expressed in JM109(DE3). |
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| Keywords: | cecropin A melittin hybrid |
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