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天蚕抗菌肽A与蜂毒素杂合肽基因的合成及在大肠杆菌中的克隆与表达
引用本文:朱嘉明,刘飞鹏,李月琴,钟振宇,张娜. 天蚕抗菌肽A与蜂毒素杂合肽基因的合成及在大肠杆菌中的克隆与表达[J]. 遗传, 2002, 24(1): 31-34
作者姓名:朱嘉明  刘飞鹏  李月琴  钟振宇  张娜
作者单位:暨南大学生命科学技术学院生物工程学系,广州 510630 Department of Biotechnology,College of Life Scienceand Technology,Jinan University,Guangzhou 510630,China
基金项目:广东省自然科学基金资助 (No .970 6 36 )
摘    要:根据天蚕抗菌肽A(cecropin A,CA)N端第1~7个 氨基酸残基、 蜂毒素(melittin,M)N端第5~12个氨基酸残基,以大肠杆菌偏爱的密码子设计合成了杂合肽CA(1~7)-M(5~12)基因,同载体pGEMEX-1连接后转化大肠杆菌JM109,经打点杂交筛选和序列测定,获得一个与设计一致的克隆。将此克隆中的质粒亚克隆至JM103(DE3) ,经IPTG诱导、BrCN切割和抑菌圈试验表明,克隆的杂合肽基因在JM109(DE3)中获得了表达。Abstract:According to the studies of David Andreu and other scientists,we designed and synthesized a gene encoding shortened cecropin A-melittin hybrid.This hybrid consists of CA(1-7) and M(5-12).The gene was inserted into pGEMEX-1 in the site of EcoRI and BamHI.A positive clone was obtained by hybridization and DNA sequencing and was expressed in JM109(DE3).

关 键 词:天蚕抗菌肽A  蜂毒素  杂合肽
Key words
  cecropin A  melittin  hybrid  
文章编号:0253-9772(2002)01-0031-04
修稿时间:2001-04-06

Cloning and Expression of a Gene Encoding Shortened Cecropin A-Melittin Hybrid in E.coli
ZHU Jia-ming,LIU Fei-peng,LI Yue-qing,ZHONG Zhen-yu,ZHANG Na. Cloning and Expression of a Gene Encoding Shortened Cecropin A-Melittin Hybrid in E.coli[J]. Hereditas, 2002, 24(1): 31-34
Authors:ZHU Jia-ming  LIU Fei-peng  LI Yue-qing  ZHONG Zhen-yu  ZHANG Na
Affiliation:Department of Biotechnology, College of Life Scienceand Technology, Jinan University, Guangzhou 510630, China. tom@jnu.edu.cn
Abstract:According to the studies of David Andreu and other scientists,we designed and synthesized a gene encoding shortened cecropin A-melittin hybrid.This hybrid consists of CA(1-7) and M(5-12).The gene was inserted into pGEMEX-1 in the site of EcoRI and BamHI.A positive clone was obtained by hybridization and DNA sequencing and was expressed in JM109(DE3).
Keywords:cecropin A  melittin  hybrid
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