甘丙肽及其受体2在抑郁症动物模型脑内的表达研究

运用慢性非预见性应激刺激建立抑郁症动物模型，用开野实验（open—field）检测大鼠建模前后行为学变化，HPLC—EC法测定血清皮质醇变化，由此对模型进行初步评价。运用原位杂交、RT—PCR方法检测大鼠海马脑区和中缝背核甘丙肽及其受体2的表达。结果显示．慢性应激后模型组大鼠活动性明显降低，血清皮质醇含量显著升高，海马脑区和中缝背核甘丙肽及其受体2的表达显著上调。在慢性应激大鼠抑郁症模型中，甘丙肽及其受体2在部分脑区的高表达提示甘丙肽很有可能参与了应激过程中神经元功能的调节。

THE EXPRESSION OF GALANIN AND GALANIN RECEPTOR-2 IN THE BRAIN OF CHRONIC STRESS MODEL OF DEPRESSION

The expression of galanin and galanin receptor-2 in hippocampus and dorsal raphe nucleus of depression model was studied. The chronic stress rat model was adopted as the modal of depression. Open-field test was used to observe the transformation of their behavior and HPLCEC was employed to analysis the level of blood serum cortisol. The method of in situ hybridization was used for testing the expression of Galanin and galanin receptor-2 in hippocampus and dorsal raphe nucleus and the method of RT-PCR was used to further analysis of the expression. The results showed that the locomotion activity decreased extremely after chronic stress, but the level of serum cortisol increased evidently. The expression of Galanin and its receptor-2 in hippocampus and dorsal raphe nucleus increased obviously. The higher expression for galanin and galanin receptor-2 in some brain area suggested that galanin probably takes part in the modulation of the function of neurons during the stress process.