Comparative study of transfection efficiency of cationic cholesterols mediated by liposomes-based gene delivery |
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Authors: | Hasegawa Seiji Hirashima Naohide Nakanishi Mamoru |
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Affiliation: | Graduate School of Pharmaceutical Sciences, Nagoya City University, Tanabe-dori, Mizuho-ku, 467-8603, Nagoya, Japan. |
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Abstract: | To develop the efficient non-viral vector for gene delivery, we compared transfection activities of cationic cholesterol derivatives. We found that the stability of the liposome-DNA complex in the presence of endosome deeply related to the transfection efficiency. We also found that the introduction of a hydrophilic group to the amino terminal of the cholesterol derivative decreased stability and facilitated the release of DNA from the endosome, resulting in higher transfection efficiency. |
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