A simple restriction fragment length polymorphism (RFLP) assay to discriminate common Porphyra (Bangiophyceae, Rhodophyta) taxa from the Northwest Atlantic |
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Authors: | Brian Teasdale Andrew West Heather Taylor Anita Klein |
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Institution: | (1) Department of Plant Biology, University of New Hampshire, Durham, 03824, USA;(2) Graduate Program in Genetics, University of New Hampshire, Durham, 03824, USA;(3) Department of Biochemistry and Molecular Biology, University of New Hampshire, Durham, 03824, USA |
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Abstract: | The identification of Porphyra species hashistorically been difficult because of the lack of distinguishing morphologicaland ecological characters. We developed a restriction fragment lengthpolymorphism (RFLP) assay, based on inter-specific sequence variation inthe ribulose bisphosphate carboxylase oxygenase largesubunit (rbcL) gene andrbcL-rbcS intergenic spacer, toprovide a simple and effective tool for screening and sorting large collectionsof Porphyra from the Northwest Atlantic. A singlerestriction digest (Hae III) discriminates betweenmultiplePorphyra species including one cryptic taxon; anadditionalenzyme (Hind III) was necessary to distinguish between theclosely related P. leucosticta and an introducedspecies P. yezoensis. |
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Keywords: | Bangiophyceae Porphyra RbcL RFLP Rhodophyta Rubisco |
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