Calmodulin-binding proteins of Saccharomyces cerevisiae |
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| Authors: | Y S Liu Y Yamashita E Tsuchiya T Miyakawa |
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| Institution: | Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Japan. |
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| Abstract: | The subcellular distribution of calmodulin-binding proteins in the soluble, plasma membrane, and nuclear fractions of Saccharomyces cerevisiae was analyzed with a gel binding assay using 125I-labeled calmodulin. Over 20 binding proteins were detected. The calmodulin-binding protein profiles were markedly different among the fractions. Calmodulin-binding proteins were most abundant in the nuclear fraction, followed by the membrane fraction and the soluble fraction in decreasing order. The amounts of certain calmodulin-binding proteins increased after treatment with alpha-mating factor. |
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